{"id":14717,"date":"2023-09-28T20:01:26","date_gmt":"2023-09-28T20:01:26","guid":{"rendered":"https:\/\/idibe.umh.es\/?page_id=14717"},"modified":"2024-07-09T10:02:23","modified_gmt":"2024-07-09T10:02:23","slug":"microscopio-confocal-lsm900-con-airyscan-2","status":"publish","type":"page","link":"https:\/\/idibe.umh.es\/en\/servicios\/microscopio-confocal-lsm900-con-airyscan-2\/","title":{"rendered":"Microscopio Confocal LSM900 con Airyscan 2"},"content":{"rendered":"

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LSM900 con Airyscan 2 Confocal Microscopy<\/h2>\n

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LSM900 with Airyscan 2 confocal microscope shows excellent features in order to acquire high resolution and quality images. Signal to noise ratio is 4 to 8 times increased and super-resolution reaches up to 120 nm.
Airyscan 2 is an area detector with an array of 32 detection elements concentrically grouped. This configuration allows the pinhole to remain open leading to the arrival of more photons and therefore increasing the efficiency. In addition, due to Airyscan 2 Multiplex mode, the acquisition speed raise significantly since with super-resolution up to four pixels of the image are scanned at the same time.
The system is equipped with a motorized high resolution XY-stage and a motorized Z-focus with 10 nm pass. Also, a motorized filter wheel with GFP, Cy3, DAPI and Cy5 filters, several 10x, 20x, 40x and 63x lenses, a LED fluorescent lighting containing 5 modules for RGB-UV, and solid-state diode laser lines of 405 nm, 488 nm, 561 nm and 640 nm are available.
Both, confocal and scanning electronic microscopes from IDiBE, can share the same kind of sample holder and use the same software. Therefore, correlative microscopy CLEM (Correlative Ligth-Electron Microscopy), can be performed in a simple way overlaying and merging information obtained by the two techniques in the same area of the sample.<\/p>\n

This equipment has been acquired thanks to an intervention co-financiated by the European Union through the\u00a0Programa Operativo del Fondo Europeo de Desarrollo Regional<\/a><\/strong><\/span>\u00a0(FEDER) and\u00a0Ministerio de Ciencia e Innovaci\u00f3n<\/a><\/span>\u00a0(Agencia Estatal de Investigaci\u00f3n)\u00a0(PROJECT:\u00a0EQC2019-005842P<\/strong>).<\/p>\n

Press\u00a0MAKE RESERVATION<\/strong>\u00a0button for further\u00a0PROCEDURES<\/strong>\u00a0and\u00a0RATES<\/strong>\u00a0information.<\/p>\n

Contact: Enrique Rodr\u00edguez (e-mail: enrique.rodriguez@umh.es<\/a>; phone:+34 965 22 2237).<\/p>\n

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Miguel Hern\u00e1ndez University, Elche
Institute of Research, Development and Innovation in Healthcare Biotechnology in Elche (IDiBE)
Torregait\u00e1n Building. Instrumentation Room 1.07 Confocal Microscopy II<\/p>\n

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LSM900 con Airyscan 2 Confocal MicroscopyLSM900 with Airyscan 2 confocal microscope shows excellent features in order to acquire high resolution and quality images. Signal to noise ratio is 4 to 8 times increased and super-resolution reaches up to 120 nm.Airyscan 2 is an area detector with an array of 32 detection elements concentrically grouped. This […]<\/p>\n","protected":false},"author":3158,"featured_media":0,"parent":1865,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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ADQUISICI\u00d3N Y AN\u00c1LISIS DE IMAGEN<\/h1>

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Microscopio Confocal LSM900 con Airyscan 2<\/h2>

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El microscopio confocal LSM900 con Airyscan 2 ofrece unas excelentes prestaciones para adquirir im\u00e1genes de la mejor calidad y resoluci\u00f3n. La relaci\u00f3n se\u00f1al ruido se ve aumentada entre 4 y 8 veces y llega a tener una superresoluci\u00f3n de hasta 120 nm.
El Airyscan 2 es un detector de \u00e1rea que cuenta con 32 elementos de detecci\u00f3n agrupados conc\u00e9ntricamente, lo que da lugar a que el \u201cpinhole\u201d pueda permanecer abierto permitiendo as\u00ed la llegada de m\u00e1s fotones e incrementando la eficiencia. Adem\u00e1s, el modo Multiplex del Airyscan 2 permite obtener hasta cuatro p\u00edxeles de la imagen con superresoluci\u00f3n en un solo barrido por lo que la velocidad de adquisici\u00f3n aumenta bastante.
Est\u00e1 equipado con una pletina motorizada en XY de alta resoluci\u00f3n y un enfoque motorizado en Z con un paso de 10nm. Adem\u00e1s, dispone de una torreta motorizada de filtros con los filtros GFP, CY3, DAPI y Cy5, de objetivos de 10x, 20x, 40x y 63x, de un sistema de iluminaci\u00f3n fluorescente LED con 5 m\u00f3dulos para RGB-UV, y de l\u00edneas l\u00e1ser tipo diodo de estado s\u00f3lido de 405 nm, 488 nm, 561 nm y 640 nm.
Tanto este microscopio confocal como el microscopio electr\u00f3nico de barrido del IDiBE pueden compartir el mismo tipo de portamuestras y hacen uso del mismo software, por lo que la microscop\u00eda correlativa CLEM (Correlative Light-Electron Microscopy) se puede llevar a cabo de una forma sencilla superponiendo y fusionando la informaci\u00f3n obtenida por ambas t\u00e9cnicas en una misma \u00e1rea de la muestra.<\/p>

El equipo ha sido adquirido gracias a una Actuaci\u00f3n cofinanciada por la Uni\u00f3n Europea a trav\u00e9s del\u00a0Programa Operativo del Fondo Europeo de Desarrollo Regional<\/a><\/strong> (FEDER) y el Ministerio de Ciencia e Innovaci\u00f3n<\/a>\u00a0(Agencia Estatal de Investigaci\u00f3n)\u00a0(PROYECTO:\u00a0EQC2019-005842P<\/strong>).<\/p>

Pulsa el bot\u00f3n RESERVA<\/strong> para m\u00e1s informaci\u00f3n sobre TASAS<\/strong> y PROCEDIMIENTOS DE TRABAJO<\/strong>.<\/p>

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Universidad Miguel Hern\u00e1ndez, Elche
Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE)
Torregait\u00e1n Building. Instrumentation Room 1.07 Confocal Microscopy II<\/p>

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