{"id":14823,"date":"2023-09-29T20:26:12","date_gmt":"2023-09-29T20:26:12","guid":{"rendered":"https:\/\/idibe.umh.es\/?page_id=14823"},"modified":"2024-07-09T07:14:18","modified_gmt":"2024-07-09T07:14:18","slug":"respirometro-oroboros-o2k","status":"publish","type":"page","link":"https:\/\/idibe.umh.es\/en\/respirometro-oroboros-o2k\/","title":{"rendered":"Respir\u00f3metro\u00a0 Oroboros O2k"},"content":{"rendered":"

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Oroboros O2k Respirometer<\/h2>\n

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A high resolution respirometer unit Oroboros O2k (Oroboros Instruments GmbH) is available in our facilities. This unit is designed to study mitochondrial physiology, including isolated mitochondria, intact or permeabilized cells and permeabilized tissues.\u00a0<\/span><\/p>\n

This equipment is able to measure both oxygen\u2019s concentration and flow at real time with very high accuracy, allowing the supply of different compounds (for instance, substrates and\/or mitochondrial inhibitors) by using Hamilton microliter syringes.\u00a0<\/span><\/p>\n

Oroboros O2k works with two respiration glass chambers with minimal oxygen diffusion: high resolution of oxygen flow: \u00b11 pmol O\u2082\/s\/mL in normoxia to anoxia; and high resolution of oxygen concentration: 5 nM. The system can work with 2 mL volumes, but is able to use 0,5 mL microchambers in order to maximize sample concentration and use the minimum possible sample quantity. Temperature is kept in a range between 4 and 47 \u00baC with high stability and precision (Peltier thermostat). In addition, the system has two PVDF shakers with electromagnetic control with the aim of maximizing the resolution while measuring oxygen consumption and an acquisition and data analysis software.<\/p>\n

Contact:<\/strong> Reinaldo Sousa dos Santos (e-mail: r.sousa@umh.es<\/a>).<\/p>\n

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This equipment has been acquired thanks to an intervention co-financiated by the European Union through the\u00a0Programa Operativo del Fondo Europeo de Desarrollo Regional<\/a><\/strong>\u00a0<\/span>(FEDER) from the\u00a0Comunitat Valenciana 2014-2020\u00a0<\/a><\/span>(PROJECT:\u00a0<\/span>IDIFEDER2020\/022<\/strong>).<\/p>\n<\/div>\n<\/div>\n

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_builder_version=”4.16″ width=”90%” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_button button_url=”http:\/\/universite.umh.es\/localizaciones\/” url_new_window=”on” button_text=”UBICACI\u00d3N” button_alignment=”center” _builder_version=”4.16″ custom_button=”on” button_text_size=”14px” button_text_color=”#ffffff” button_bg_color=”#c0172d” button_border_width=”0px” button_border_radius=”3px” button_letter_spacing=”1px” button_font=”Raleway|600||on|||||” button_use_icon=”off” background_layout=”dark” custom_padding=”10px|30px|10px|30px” animation_style=”fade” button_letter_spacing_hover=”1px” global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” 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Torrepinet Building. Microscopy Unit (Room E08P0008)” _builder_version=”4.16″ width=”60%” custom_margin=”||3px” custom_padding=”||0px” border_radii=”on|4px|4px|4px|4px” locked=”off” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.16″ text_font=”||||||||” text_text_color=”#000000″ text_font_size=”16px” text_text_align=”left” global_colors_info=”{}”]<\/p>\n

Miguel Hern\u00e1ndez University, Elche
Institute for Research, Development and Innovation in Health Biotechnology of Elche (IDiBE).
Torregait\u00e1n Building. Instrumentation room 1.02 Histochemistry<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Oroboros O2k Respirometer A high resolution respirometer unit Oroboros O2k (Oroboros Instruments GmbH) is available in our facilities. This unit is designed to study mitochondrial physiology, including isolated mitochondria, intact or permeabilized cells and permeabilized tissues.\u00a0 This equipment is able to measure both oxygen\u2019s concentration and flow at real time with very high accuracy, allowing […]<\/p>\n","protected":false},"author":3158,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

[et_pb_section fb_built=\"1\" _builder_version=\"4.16\" use_background_color_gradient=\"on\" background_color_gradient_start=\"rgba(255,255,255,0.82)\" background_color_gradient_end=\"rgba(255,255,255,0.74)\" background_color_gradient_overlays_image=\"on\" background_image=\"https:\/\/idibe.es\/wp-content\/uploads\/2019\/03\/laboratory-3827742_1920.jpg\" custom_padding=\"54px|0px|18px|0px|false|false\" locked=\"off\"][et_pb_row _builder_version=\"4.16\" custom_padding=\"28px|0px|29px|0px|false|false\"][et_pb_column type=\"4_4\" _builder_version=\"4.16\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_text _builder_version=\"4.16\" header_font=\"||||||||\" header_font_size=\"55px\"]<\/p>

INSTALACIONES DE ELECTROFISIOLOG\u00cdA<\/h1>

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\"1\" _builder_version=\"4.16\" background_color=\"#c0172d\" custom_padding=\"16px|0px|16px|0px|false|false\" locked=\"off\"][et_pb_row _builder_version=\"4.16\" custom_padding=\"11px|0px|0|0px|false|false\"][et_pb_column type=\"4_4\" _builder_version=\"4.16\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_text _builder_version=\"4.16\" header_font=\"||||||||\" header_font_size=\"55px\" header_2_font=\"Raleway||||||||\" header_2_text_color=\"#ffffff\" header_2_font_size=\"45px\" header_3_font=\"||||||||\" header_4_font=\"||||||||\" header_4_text_color=\"#ffffff\"]<\/p>

Respir\u00f3metro\u00a0 Oroboros O2k<\/h2>

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\"1\" _builder_version=\"4.16\" locked=\"off\"][et_pb_row _builder_version=\"4.16\" background_size=\"initial\" background_position=\"top_left\" background_repeat=\"repeat\"][et_pb_column type=\"4_4\" _builder_version=\"4.16\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_image src=\"https:\/\/idibe.es\/wp-content\/uploads\/2021\/02\/IDIFEDER2020_022_CMOS-MEA500_IDiBE.png\" align=\"center\" _builder_version=\"4.16\" width=\"79%\" module_alignment=\"center\"][\/et_pb_image][et_pb_image src=\"https:\/\/idibe.umh.es\/files\/2023\/09\/Oroboros-O2k_IDIFEDER-2020_22_IDiBE-2021.png\" align=\"center\" _builder_version=\"4.16\" width=\"79%\" module_alignment=\"center\" locked=\"off\"][\/et_pb_image][et_pb_text _builder_version=\"4.16\" text_font=\"||||||||\" header_font=\"||||||||\" header_4_font=\"||||||||\" width=\"90%\" custom_margin=\"|||64px\"]Disponemos de una unidad de respirometr\u00eda de alta resoluci\u00f3n modelo Oroboros O2k de Oroboros Instruments GmbH. Esta unidad sirve para el estudio de la fisiolog\u00eda mitocondrial, incluyendo su uso utilizando mitocondrias aisladas, c\u00e9lulas (intactas o permeabilizadas) y tejidos permeabilizados. El equipo mide tanto la concentraci\u00f3n como el flujo de ox\u00edgeno con muy alta precisi\u00f3n en tiempo real, permitiendo la inyecci\u00f3n de distintos compuestos (sustratos y\/o inhibidores mitocondriales, por ejemplo) mediante uso de microjeringas Hamilton.<\/p>

\u00a0<\/p>

El equipo cuenta con dos c\u00e1maras de respiraci\u00f3n con m\u00ednima difusi\u00f3n de ox\u00edgeno: alta resoluci\u00f3n del flujo de ox\u00edgeno: \u00b1 1 pmol O\u2082\/s\/mL en normoxia a anoxia; alta resoluci\u00f3n de concentraci\u00f3n de ox\u00edgeno: 5 nM. Las c\u00e1maras de vidrio permiten trabajar con vol\u00famenes de 2 ml, pero existe la posibilidad de utilizar microc\u00e1maras de 0,5 ml para maximizar las concentraciones de muestra y poder trabajar con la menor cantidad de muestra posible. El sistema mantiene la temperatura en un rango entre 4 y 47 \u00baC con alta estabilidad y precisi\u00f3n (termostato Peltier). El equipo cuenta con agitadores de PVDF con control electromagn\u00e9tico a fin de maximizar la resoluci\u00f3n en la medida de consumo de ox\u00edgeno y un software para adquisici\u00f3n y an\u00e1lisis de datos.<\/p>

Este equipo ha sido adquirido gracias a una Actuaci\u00f3n cofinanciada por la Uni\u00f3n Europea a trav\u00e9s del Programa Operativo del Fondo Europeo de Desarrollo Regional<\/a><\/strong>\u00a0(FEDER) from the\u00a0Comunitat Valenciana 2014-2020<\/a>\u00a0(PROYECTO:\u00a0IDIFEDER2020\/022<\/strong>).<\/p>

El equipo ha sido adquirido gracias a una Actuaci\u00f3n cofinanciada por la Uni\u00f3n Europea a trav\u00e9s del\u00a0Programa Operativo del Fondo Europeo de Desarrollo Regional<\/a><\/strong>\u00a0(FEDER) de la\u00a0Comunitat Valenciana 2014-2020\u00a0<\/a>(PROYECTO:\u00a0IDIFEDER2018\/020<\/strong>).[\/et_pb_text][et_pb_image src=\"https:\/\/idibe.umh.es\/files\/2023\/09\/Placa-IDIFEDER-2020_022_-OROBOROS_IDiBE-2021.png\" align=\"center\" _builder_version=\"4.16\" width=\"70%\"][\/et_pb_image][et_pb_image src=\"https:\/\/idibe.es\/wp-content\/uploads\/2021\/02\/Placa-IDIFEDER2020_022_CMOS-MEA500.png\" align=\"center\" _builder_version=\"4.16\" width=\"60%\"][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=\"4.16\" custom_padding=\"0|0px|1px|0px|false|false\"][et_pb_column type=\"4_4\" _builder_version=\"4.16\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_divider divider_weight=\"3px\" _builder_version=\"4.16\" max_width=\"17%\" 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Universidad Miguel Hern\u00e1ndez, Elche
Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE)
Edificio Torregait\u00e1n. Sala de Instrumentaci\u00f3n 1.02 Histoqu\u00edmica<\/p>

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