{"id":14827,"date":"2023-09-29T21:02:36","date_gmt":"2023-09-29T21:02:36","guid":{"rendered":"https:\/\/idibe.umh.es\/?page_id=14827"},"modified":"2024-07-09T10:07:36","modified_gmt":"2024-07-09T10:07:36","slug":"bd-facscanto-ii","status":"publish","type":"page","link":"https:\/\/idibe.umh.es\/en\/servicios\/bd-facscanto-ii\/","title":{"rendered":"BD FACSCANTO II"},"content":{"rendered":"

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BD FACSCANTO II<\/h2>\n

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The FACSCanto II cytometer is a fluidics system coupled with advanced optics that allows for the characterization of cells\/particles in suspension (recommended maximum particle size: 50 \u00b5m) according to their ability to scatter or emit light when exposed to light sources (lasers) at specific wavelengths. The resulting data is collected for multiple parameters for each individual cell at high speed (up to thousands of cells\/particles per second). For example, the cell cycle, multiple immune cell populations and phenotypes or the expression of a fluorescent reporter can be assessed and quantified accurately for a large number of cells in minutes or even seconds.<\/p>\n

The optical setup the FACSCanto II consists of three different lasers: 405 nm (violet), 488 nm (blue) and 633 nm (red). The fluorescence emission is filtered (see below) and then amplified by 8 photomultiplier tube detectors. Examples of fluorophores and fluorescent proteins suitable for studies using the following laser-filter combinations available in the FACSCanto II are indicated in brackets.<\/p>\n

Wavelength ranges detected from 488-nm laser:
\u2022 750-810 nm (e.g., PE-Cy7)
\u2022 670-735 nm (PerPC-Cy5.5)
\u2022 564-606 nm (PE)
\u2022 515-545 nm (FITC, GFP)<\/p>\n

Wavelength ranges detected from 633-nm laser:
\u2022 750-810 nm (APC-Cy7)
\u2022 650-670 nm (APC)<\/p>\n

Wavelength ranges detected from 405-nm laser:
\u2022 502-535 nm (AmCyan)
\u2022 425-475 nm (DAPI, Pacific Blue \u00ae)<\/p>\n

Forward scatter detection: Photodiode with 488\/10 bandpass filter
Side scatter detection: PMT with 488\/10 bandpass filter<\/p>\n

Common applications of this apparatus include:<\/p>\n