{"id":16629,"date":"2024-03-11T11:29:32","date_gmt":"2024-03-11T11:29:32","guid":{"rendered":"https:\/\/idibe.umh.es\/?page_id=16629"},"modified":"2024-07-09T07:44:01","modified_gmt":"2024-07-09T07:44:01","slug":"biosafety-laboratory-level-2","status":"publish","type":"page","link":"https:\/\/idibe.umh.es\/en\/servicios\/biosafety-laboratory-level-2\/","title":{"rendered":"Biosafety Laboratory Level 2"},"content":{"rendered":"

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Biosafety Laboratory Level 2<\/h2>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ locked=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/idibe.umh.es\/files\/2024\/02\/WhatsApp-Image-2024-02-27-at-13.08.36-226×300.jpeg” title_text=”WhatsApp Image 2024-02-27 at 13.08.36″ align=”center” _builder_version=”4.17.4″ width=”79%” module_alignment=”center” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.16″ text_font=”||||||||” text_text_color=”#000000″ text_font_size=”16px” header_font=”||||||||” header_4_font=”||||||||” text_orientation=”justified” width=”90%” custom_margin=”|||64px” global_colors_info=”{}”]<\/p>\n

The biosafety laboratory level 2 (LBS2) of the IDiBE is a facility for the safe culture of pathogenic bacteria and viruses. The pathogens intended for use in this facility are agents of moderate potential hazard to personnel and the environment.<\/p>\n

A biosafety laboratory is a biological containment facility that has a series of measures concerning facilities, equipment and personnel organisation to isolate hazardous biological agents from laboratory operators and from outside the laboratory (including the environment). The containment levels and measures are established according to the level of risk posed by working with biological agents. Specifically, the IDiBE LBS2 is designed for work with pathogenic biological agents included in risk group 2, i.e. those biological agents that can cause illness in humans and can pose a danger to workers, but are unlikely to spread to the community and effective prophylaxis or treatment is generally available.<\/p>\n

The containment measures include a unique ventilation system that pressurises the work rooms to prevent the possible escape of contaminants to the outside, as well as an air treatment unit with HEPA filters to filter the extracted air before it is released into the atmosphere.<\/p>\n

This laboratory consists of an anteroom and two workrooms for working with viruses and bacteria, respectively. Each of these work rooms is equipped with specific incubators for the cultivation of bacteria or cell lines and with type II biological safety cabinets (EN 12469:2000), designed to protect the user, the samples and the environment.<\/p>\n

This new facility will give the Institute the capacity to undertake new lines of research and more ambitious and competitive projects that require this level of Biosafety. For example, one of the projects will include the testing of new vaccines against respiratory syncytial virus (RSV), in collaboration with the Hospital General Universitario de Elche.<\/p>\n

Entry and use of this facility is subject to prior authorisation by the university’s scientific and occupational risk managers and the number of regular users will be restricted.<\/p>\n

The main researcher responsible for these facilities is Dr. Mar\u00eda del Mar Ortega-Villaiz\u00e1n and the technician responsible is Dr. Mar\u00eda Lourdes Renart.<\/p>\n

Contact<\/strong>: Maria Lourdes Renart P\u00e9rez (e-mail: lrenart@umh.es;<\/a>\u00a0 phone: +34 648 891 376).<\/p>\n

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Torrepinet Building. Microscopy Unit (Room E08P0008)” _builder_version=”4.16″ width=”60%” custom_margin=”||3px” custom_padding=”||0px” border_radii=”on|4px|4px|4px|4px” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.16″ text_font=”||||||||” text_text_color=”#000000″ text_font_size=”16px” text_text_align=”left” global_colors_info=”{}”]<\/p>\n

Miguel Hern\u00e1ndez University, Elche
\nInstitute of Research, Development and Innovation in Healthcare Biotechnology in Elche (IDiBE)
\nTorregait\u00e1n Building. Intrumentation Room 0.07 Chromatography<\/p>\n

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CONTACTA CON NOSOTROS<\/span><\/span><\/h2>\n

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\nEdificio Torregait\u00e1n.
\n03202 Elche, Alicante, Spain
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S\u00cdGUENOS<\/h2>\n

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Biosafety Laboratory Level 2The biosafety laboratory level 2 (LBS2) of the IDiBE is a facility for the safe culture of pathogenic bacteria and viruses. The pathogens intended for use in this facility are agents of moderate potential hazard to personnel and the environment. A biosafety laboratory is a biological containment facility that has a series […]<\/p>\n","protected":false},"author":16956,"featured_media":0,"parent":1865,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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INSTALACIONES ANAL\u00cdTICAS<\/h1>

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UHPLC\/MS Triple Cuadrupolo<\/h2>

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Sistema de Cromatograf\u00eda L\u00edquida de Ultra Alta Presi\u00f3n (uHPLC) Shimadzu, de la serie NEXERA, acoplado a un detector de espectrometr\u00eda de masas con triple cuadrupolo modelo LCMS-8050 para masas en t\u00e1ndem (QqQ-MS\/MS). Los equipos de uHPLC permiten acelerar los procedimientos de cromatograf\u00eda l\u00edquida, incrementar la productividad y disminuir el consumo de fase m\u00f3vil reduciendo los costes y p\u00e9rdidas, con las consiguientes ventajas en el desarrollo de procesos anal\u00edticos en los campos farmac\u00e9utico, alimentario y cosm\u00e9tico. El acoplamiento de este cromat\u00f3grafo l\u00edquido a un detector de espectrometr\u00eda de masas de QqQ de alta velocidad de barrido (>15.000 umas\/s) permite el desarrollo de m\u00e9todos altamente robustos y reproducibles, reducir los tiempos de an\u00e1lisis frente a los m\u00e9todos convencionales, debido a las elevadas corrientes de iones generadas, y alcanzar una alta sensibilidad en modo MRM (multiple reaction monitoring).<\/p>

La sensibilidad de este equipo permite alcanzar un l\u00edmite de detecci\u00f3n del orden del picogramo en modo MRM, lo que le hace especialmente indicado para el an\u00e1lisis cuantitativo de todo tipo de mol\u00e9culas: compuestos org\u00e1nicos, metabolitos, p\u00e9ptidos tr\u00edpticos, aditivos, f\u00e1rmacos, pesticidas, etc. Por otra parte, este equipo tambi\u00e9n ofrece resoluciones por debajo de la unidad de masa (R < 0.7 FWHM) para la identificaci\u00f3n de compuestos, adem\u00e1s de la posibilidad de realizaci\u00f3n de masas en t\u00e1ndem para an\u00e1lisis estructural. Entre las m\u00faltiples aplicaciones de este equipo se encuentran:<\/p>