{"id":3310,"date":"2023-09-08T12:29:53","date_gmt":"2023-09-08T12:29:53","guid":{"rendered":"https:\/\/idibe.umh.es\/?page_id=3310"},"modified":"2025-04-11T10:12:00","modified_gmt":"2025-04-11T10:12:00","slug":"citometro-de-masas-acoplado-a-sistema-de-imagen-helios-hyperion","status":"publish","type":"page","link":"https:\/\/idibe.umh.es\/en\/citometro-de-masas-acoplado-a-sistema-de-imagen-helios-hyperion\/","title":{"rendered":"Cit\u00f3metro de masas acoplado a sistema de imagen Helios Hyperion"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.17.4″ _module_preset=”default” background_color=”#bc2a30″ custom_padding=”16px|0px|16px|0px|true|true” global_colors_info=”{}”][et_pb_row _builder_version=”4.17.4″ _module_preset=”default” custom_padding=”11px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.17.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.17.4″ text_font=”Verdana|700|||||||” text_font_size=”30px” header_font=”Raleway||||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”45px” header_line_height=”1.1em” header_2_font=”Raleway|||on|||||” header_2_text_color=”#003041″ header_2_font_size=”54px” global_colors_info=”{}”]<\/p>\n

CyTOF XT mass cytometer coupled to Hyperion XTi imaging system<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ background_enable_color=”off” custom_padding=”||4px|||” locked=”off” global_colors_info=”{}”][et_pb_row admin_label=”row” _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_padding=”||19px|||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.17.4″ text_text_color=”#000000″ text_font_size=”20px” text_line_height=”1.6em” text_orientation=”justified” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n

IDiBE features a mass cytometer (CyTOF XT) which is complemented by an imaging system (Hyperion XTi)<\/strong> from Standard BioTools.<\/p>\n

The CyTOF XT allows the analysis of single cells labelled with stable metal isotopes using advanced inductively coupled plasma technology. With 135 detection channels, this instrument is capable of simultaneous multiple markers, maximising the information obtained per cell.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_5,3_5,1_5″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_column type=”1_5″ _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_column][et_pb_column type=”3_5″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_image src=”https:\/\/idibe.umh.es\/files\/2025\/04\/Helios-XTI.jpg” _builder_version=”4.17.4″ hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″ title_text=”Helios XTI”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_5″ _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][et_pb_row admin_label=”row” _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_padding=”||19px|||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.17.4″ text_text_color=”#000000″ text_font_size=”20px” text_line_height=”1.6em” text_orientation=”justified” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n

<\/span><\/p>\n

This system can be coupled to the Hyperion XTi high-throughput imaging system, capable of analysing more than 40 protein markers simultaneously. The use of these metallic markers enables a spatial resolution of 1 \u00b5m in tissues and cell smears, resulting in high spatial and parametric definition of cells in situ.<\/p>\n\n\n\n\n\n\n\n\n\n\n
\n

 <\/p>\n<\/td>\n

\n

CyTOF XT<\/strong><\/p>\n<\/td>\n

\n

Hyperion XTi<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Mass range\u00a0<\/strong><\/p>\n<\/td>\n

\n

75 \u2013 209 uma<\/p>\n<\/td>\n

\n

75 \u2013 209 uma<\/p>\n<\/td>\n<\/tr>\n

\n

Channels<\/strong><\/p>\n<\/td>\n

\n

135<\/p>\n<\/td>\n

\n

135<\/p>\n<\/td>\n<\/tr>\n

\n

Simultaneous markers<\/strong><\/p>\n<\/td>\n

\n

>45<\/p>\n<\/td>\n

\n

>40<\/p>\n<\/td>\n<\/tr>\n

\n

Sample format<\/strong><\/p>\n<\/td>\n

\n

Pellet or suspension<\/p>\n<\/td>\n

\n

Cell monolayer 4-7 \u00b5m<\/p>\n<\/td>\n<\/tr>\n

\n

Throughput\u00a0<\/strong><\/p>\n<\/td>\n

\n

500 events\/second<\/p>\n<\/td>\n

\n

800 pixels\/second<\/p>\n<\/td>\n<\/tr>\n

\n

Spatial resolution<\/strong><\/p>\n<\/td>\n

\n

 <\/p>\n<\/td>\n

\n

Preview Mode<\/u>: 25 \u00b5m<\/p>\n

Tissue Mode<\/u>: 5 \u00b5m<\/p>\n

Cell Mode<\/u>: 1 \u00b5m<\/p>\n<\/td>\n<\/tr>\n

\n

Instrument response\u00a0<\/strong><\/p>\n<\/td>\n

\n

500 Tb159 <\/sup>dual counts\/EQTM <\/sup>Six Element Calibration Bead<\/p>\n<\/td>\n

\n

Preview Mode<\/u>: \u2265700mm2<\/sup>\/h<\/p>\n

Tissue Mode<\/u>: \u226550mm2<\/sup>\/h<\/p>\n

Cell Mode<\/u>: \u22652mm2<\/sup>\/h<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/et_pb_text][et_pb_text _builder_version=”4.16″ text_text_color=”#000000″ text_font_size=”20px” text_line_height=”2em” text_orientation=”justified” global_colors_info=”{}”]<\/p>\n

The acquisition of this equipment has been possible thanks to the Grant EQC2021-007485-P<\/strong> funded by MICIU\/AEI\/10.13039\/501100011033 and the \u201cEuropean Union NextGenerationEU\/PRTR\u201d.<\/span><\/p>\n

Contact:<\/strong> Marta Rubio Camacho (e-mail: marta.rubioc@umh.es<\/a>; phone: +34 965 22 26 88)<\/span><\/p>\n

\n

[\/et_pb_text][et_pb_image src=”https:\/\/idibe.umh.es\/files\/2024\/10\/Imagen-Hyperion-1.png” _builder_version=”4.17.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

CyTOF XT mass cytometer coupled to Hyperion XTi imaging systemIDiBE features a mass cytometer (CyTOF XT) which is complemented by an imaging system (Hyperion XTi) from Standard BioTools. The CyTOF XT allows the analysis of single cells labelled with stable metal isotopes using advanced inductively coupled plasma technology. With 135 detection channels, this instrument is […]<\/p>\n","protected":false},"author":3158,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

[et_pb_section fb_built=\"1\" admin_label=\"section\" _builder_version=\"4.2.2\" background_enable_color=\"off\" custom_padding=\"||4px|||\" locked=\"off\"][et_pb_row admin_label=\"row\" _builder_version=\"4.2.2\" background_size=\"initial\" background_position=\"top_left\" background_repeat=\"repeat\" custom_padding=\"||19px|||\"][et_pb_column type=\"4_4\" _builder_version=\"3.25\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_text _builder_version=\"4.2.2\" text_font=\"Verdana|700|||||||\" text_font_size=\"30px\" header_font=\"|600|||||||\" header_font_size=\"44px\" header_line_height=\"1.1em\" header_2_font=\"|700||on|||||\" header_2_text_color=\"#003041\" header_2_font_size=\"54px\"]<\/p>

Cit\u00f3metro de masas acoplado a sistema de imagen Helios Hyperion<\/span><\/h1>

[\/et_pb_text][et_pb_text _builder_version=\"4.2.2\" text_text_color=\"#000000\" text_font_size=\"20px\" text_line_height=\"1.6em\" text_orientation=\"justified\"]<\/p>

The IDiBE has a <\/span>Helios Mass Cytometer<\/b> (CyTOF) which is complemented by a <\/span>Hyperion+ Imaging System<\/b> (Hyperion Tissue Image) from Standard BioTools.\u00a0<\/span><\/p>

Helios allows the analysis of single cells labeled with stable metal isotopes using state-of-the-art inductively coupled plasma technology. With 135 detection channels, this equipment is capable of simultaneously detecting multiple markers, maximizing the information obtained per cell.\u00a0<\/span><\/p>

This system can be coupled to the high-throughput imaging system; the Hyperion+, which is capable of analyzing more than 40 protein markers simultaneously. The use of these metallic markers allows a spatial resolution of 1 \u00b5m in tissues and cell smears, resulting in a spatial and parametric definition of cells <\/span>in situ<\/span><\/i>.<\/span><\/p>

[\/et_pb_text][et_pb_image src=\"https:\/\/idibe.umh.es\/files\/2023\/09\/tabla-citometro.png\" align=\"center\" _builder_version=\"4.2.2\"][\/et_pb_image][et_pb_text _builder_version=\"4.2.2\" text_text_color=\"#000000\" text_font_size=\"20px\" text_line_height=\"2em\" text_orientation=\"justified\"]<\/p>

The acquisition of this equipment has been possible thanks to a <\/span>grant for the acquisition of Scientific-Technical Equipment<\/b> co-funded by the <\/span>Ministry of Science and Innovation<\/span><\/i>, the <\/span>State Research Agency<\/span><\/i> and by <\/span>the European Union NextGenerationEU\/Plan for the Recovery, Transformation and Resilience of Spain<\/span><\/i> (<\/span>PROJECT: EQC2021-007485-P<\/b>).<\/span><\/p>

For more information on RATES and WORKING PROTOCOLS, click on the +INFO button.\u00a0<\/span><\/p>

Contact: Marta Rubio Camacho (e-mail: marta.rubioc@umh.es<\/a>; phone: 965 22 26 88)<\/span><\/p>

[\/et_pb_text][et_pb_image src=\"https:\/\/idibe.umh.es\/files\/2023\/09\/financiacion-ministerio-ciencia-innovacion.png\" align=\"center\" _builder_version=\"4.2.2\"][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","_et_gb_content_width":"","_links_to":"","_links_to_target":""},"_links":{"self":[{"href":"https:\/\/idibe.umh.es\/en\/wp-json\/wp\/v2\/pages\/3310"}],"collection":[{"href":"https:\/\/idibe.umh.es\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/idibe.umh.es\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/idibe.umh.es\/en\/wp-json\/wp\/v2\/users\/3158"}],"replies":[{"embeddable":true,"href":"https:\/\/idibe.umh.es\/en\/wp-json\/wp\/v2\/comments?post=3310"}],"version-history":[{"count":0,"href":"https:\/\/idibe.umh.es\/en\/wp-json\/wp\/v2\/pages\/3310\/revisions"}],"wp:attachment":[{"href":"https:\/\/idibe.umh.es\/en\/wp-json\/wp\/v2\/media?parent=3310"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}