{"id":3757,"date":"2023-09-25T10:14:03","date_gmt":"2023-09-25T10:14:03","guid":{"rendered":"https:\/\/idibe.umh.es\/?page_id=3757"},"modified":"2024-10-14T12:11:10","modified_gmt":"2024-10-14T12:11:10","slug":"bioimpresora-3d-bs3-3-gesim","status":"publish","type":"page","link":"https:\/\/idibe.umh.es\/en\/servicios\/bioimpresora-3d-bs3-3-gesim\/","title":{"rendered":"Bioimpresora 3D (BS3.3 GeSiM)"},"content":{"rendered":"

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GeSIM 3D Bioprinter (BS3.3 GeSiM)<\/h2>\n

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The bioprinter is a versatile equipment designed to tackle current problems in cutting-edge research fields and, therefore, it has many tools that allow extruding a huge range of polymers such as those employed in tissue engineering. A high flexibility and compact dimensions are combined so it could be used in biological safety cabinets and sterile environments.<\/span><\/p>\n

A cartesian robotized platform controls the movement of the bioprinter in X, Y and Z axes. In the horizontal XY-plane it has a range of 385 mm x 265 mm with micrometric precision. Several tools are located in four independent Z-axes at the same time with a range of 100 mm.<\/span><\/p>\n

In the first 3 Z-axes different holders are located for pneumatic extrusion. Several adaptors make possible to use 10 and 30 mL cartridges with nozzles whose diameters range from 250 to 840 \u00b5m. Besides, a peltier module is available to regulate temperature from 4 to 80\u00b0C in a metallic cartridge with nozzle diameters from 150 to 330 \u00b5m. In addition, another adapter allows housing a 50 mL syringe driven by a step motor. A core\/shell extruder for coaxial printing of two materials and a 365 nm UV-LED pen are also available.<\/span><\/p>\n

The fourth Z-axis holds a Z-sensor and a piezoelectric pipet. The former measures the height of the printing points and the latter, which is pneumatically controlled, transfers small liquid sample amounts, around 300 pL droplets, from multi-well plates to a defined location with a frequency of around 1000 droplets per second.<\/span><\/p>\n

The equipment also has several accessories to facilitate the extrusion such as stroboscopic light to measure the diameter of the nozzles or the shape and size of the piezoelectric pipet droplets, a wash station and a dry pad also for the pipet, or cleaning brushes ant a tip cleaner for the plastic or metallic nozzles. On top of that, other accessories might be available increasing the potential of the equipment.<\/span><\/p>\n

The great versatility of this bioprinter, its modular configuration and the huge range of tools and accessories which are available, make it essential in a big amount of applications like printing different (bio-)polymers which might contain cells, the design or manufacturing of multi-material scaffolds for tissue engineering, organoid printing, co-printing of hard and soft biopolymers, functionalization of metal implants with soft polymer layers, etcetera.<\/span><\/p>\n

This equipment has been acquired thanks to an intervention co-financiated by the European Union through the Programa Operativo del Fondo Europeo de Desarrollo Regional (FEDER) from the Comunitat Valenciana 2014-2020 (PROYECTO: IDIFEDER2020\/022).<\/span><\/p>\n

Contact: Enrique Rodr\u00edguez<\/strong> (e-mail: enrique.rodriguez@umh.es<\/a>; phone:+34 965 22 2237).<\/span><\/p>\n

<\/span><\/p>\n

[\/et_pb_text][et_pb_image src=”https:\/\/idibe.umh.es\/files\/2023\/09\/FEDER-Bioimpresora-3D_IDiBE-2022.png” align=”center” _builder_version=”4.16″ width=”60%” locked=”off” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_5,3_5,1_5″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_column type=”1_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_button button_url=”https:\/\/calendar.google.com\/calendar\/embed?src=fesem.idibe%40umh.es&ctz=Europe%2FMadrid” url_new_window=”on” button_text=”DISPONIBILIDAD” button_alignment=”center” disabled_on=”on|on|on” _builder_version=”4.16″ custom_button=”on” button_text_size=”14px” button_text_color=”#ffffff” button_bg_color=”#c0172d” button_border_width=”0px” button_border_radius=”3px” button_letter_spacing=”1px” button_font=”Raleway|600||on|||||” button_use_icon=”off” background_layout=”dark” custom_padding=”10px|30px|10px|30px” animation_style=”fade” button_letter_spacing_hover=”1px” disabled=”on” global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”on” button_letter_spacing__hover=”1px” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_button][et_pb_image src=”https:\/\/idibe.umh.es\/files\/2019\/02\/Building-2.png” _builder_version=”4.16″ width=”90%” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”3_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/idibe.umh.es\/files\/2019\/02\/Research_V01-copia.png” _builder_version=”4.16″ width=”90%” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/idibe.umh.es\/files\/2020\/05\/Torregaitan-IDiBE-equipos.jpg” align=”center” admin_label=”Miguel Hern\u00e1ndez University, Elche. 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Miguel Hern\u00e1ndez University, Elche<\/span>
Institute of Research, Development and Innovation in Healthcare Biotechnology in Elche (IDiBE)<\/span>
Torregait\u00e1n Building. Bioprinting Unit. E10P1047<\/span><\/p>\n

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GeSIM 3D Bioprinter (BS3.3 GeSiM)The bioprinter is a versatile equipment designed to tackle current problems in cutting-edge research fields and, therefore, it has many tools that allow extruding a huge range of polymers such as those employed in tissue engineering. A high flexibility and compact dimensions are combined so it could be used in biological […]<\/p>\n","protected":false},"author":3158,"featured_media":0,"parent":1865,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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UNIDAD DE CULTIVOS CELULARES<\/h1>

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\"1\" _builder_version=\"4.16\" background_color=\"#c0172d\" custom_padding=\"16px|0px|16px|0px|false|false\" locked=\"off\"][et_pb_row _builder_version=\"4.16\" custom_padding=\"11px|0px|0|0px|false|false\"][et_pb_column type=\"4_4\" _builder_version=\"4.16\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_text _builder_version=\"4.2.2\" header_font=\"||||||||\" header_font_size=\"55px\" header_2_font=\"Raleway||||||||\" header_2_text_color=\"#ffffff\" header_2_font_size=\"45px\" header_3_font=\"||||||||\" header_4_font=\"||||||||\" header_4_text_color=\"#ffffff\"]<\/p>

Bioimpresora 3D (BS3.3 GeSiM)<\/h2>

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\"1\" _builder_version=\"4.16\" locked=\"off\"][et_pb_row _builder_version=\"4.16\" background_size=\"initial\" background_position=\"top_left\" background_repeat=\"repeat\"][et_pb_column type=\"4_4\" _builder_version=\"4.16\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_image src=\"https:\/\/idibe.umh.es\/files\/2023\/09\/Bioimpresora-3D_IDIBE-2022-1.png\" align=\"center\" _builder_version=\"4.16\" width=\"79%\" module_alignment=\"center\" locked=\"off\"][\/et_pb_image][et_pb_text _builder_version=\"4.16\" text_font=\"||||||||\" header_font=\"||||||||\" width=\"90%\" custom_margin=\"|||64px\"]<\/p>

El equipo consiste en una bioimpresora configurable dise\u00f1ada con el prop\u00f3sito de ser una herramienta que aborde problemas actuales en campos de investigaci\u00f3n punteros. La variedad de herramientas de dispensaci\u00f3n permite la impresi\u00f3n de gran cantidad de pol\u00edmeros como los empleados en ingenier\u00eda de tejidos. Combina una alta flexibilidad de configuraci\u00f3n con dimensiones compactas lo que permiten su uso en cabinas de seguridad y ambientes est\u00e9riles.<\/p>

Se trata de una plataforma robotizada en los tres ejes cartesianos X, Y, y Z<\/strong>. En el plano horizontal XY puede recorrer un rango de 385 mm x 265 mm con precisi\u00f3n microm\u00e9trica<\/strong>. Dispone de hasta 4 ejes Z independientes<\/strong> donde se pueden colocar varias herramientas al mismo tiempo con un recorrido de unos 100 mm.<\/p>

En los tres primeros ejes Z se pueden alojar distintos dispensadores<\/strong>. Existen adaptadores para colocar cartuchos de 10 ml y 30 mL, controlados de forma neum\u00e1tica con boquillas de diversos tama\u00f1os desde 250 a 840 \u00b5m. Tambi\u00e9n se dispone de un m\u00f3dulo peltier<\/strong> asociado a un cartucho met\u00e1lico cuya temperatura puede ajustarse entre 4 y 80 \u00b0C, con boquillas de entre 150 y 330 \u00b5m. Adem\u00e1s, se puede acoplar un m\u00f3dulo para una jeringa de 50 ml accionado de forma el\u00e9ctrica. Tambi\u00e9n cuenta con un dispensador doble coaxial<\/strong> que permite extruir dos materiales al mismo tiempo, uno encapsulado dentro del otro. Otro accesorio que se puede acoplar es una luz ultravioleta<\/strong> de 365 nm.<\/p>

El cuarto eje Z aloja un sensor de altura<\/strong> y una pipeta piezoel\u00e9ctrica<\/strong>. El primero mide la altura de los puntos donde se va a imprimir y la segunda, que est\u00e1 controlada de forma neum\u00e1tica, es capaz de aspirar sustancias de placas multipocillo y depositar gotas de unos 300 pL en una posici\u00f3n determinada con una frecuencia de hasta 1000 gotas por segundo.<\/p>

La plataforma tambi\u00e9n cuenta con diversos accesorios<\/strong> para facilitar la extrusi\u00f3n como luz estrobosc\u00f3pica para medir el tama\u00f1o de las boquillas o medir el tama\u00f1o de gota de la pipeta piezoel\u00e9ctrica, una estaci\u00f3n de lavado y otra de secado para la pipeta piezoel\u00e9ctrica, o estaciones de limpieza con cepillos para las boquillas de pl\u00e1stico o met\u00e1licas. La plataforma dispone de otros accesorios que se pueden adquirir aumentando as\u00ed el potencial del equipo.<\/p>

La gran versatilidad de la bioimpresora, su configuraci\u00f3n modular y la amplia variedad de accesorios y herramientas de que dispone la hacen esencial en muchas aplicaciones entre las que destacan la impresi\u00f3n de distintos (bio-)pol\u00edmeros que pueden contener c\u00e9lulas, el dise\u00f1o y fabricaci\u00f3n de estructuras multimaterial para ingenier\u00eda de tejidos, la impresi\u00f3n de organoides, la impresi\u00f3n conjunta de biopol\u00edmeros duros o blandos, o la funcionalizaci\u00f3n de implantes met\u00e1licos con capas blandas.<\/p>

El equipo ha sido adquirido gracias a una actuaci\u00f3n cofinanciada por la Uni\u00f3n Europea a trav\u00e9s del Programa Operativo del Fondo Europeo de Desarrollo Regional (FEDER) de la Comunitat Valenciana 2014-2020 (PROYECTO: IDIFEDER2020\/022).<\/p>

Pulsa el bot\u00f3n\u00a0RESERVA<\/strong>\u00a0para m\u00e1s informaci\u00f3n sobre\u00a0TASAS<\/strong>\u00a0y\u00a0PROCEDIMIENTOS DE TRABAJO.<\/strong><\/p>

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Universidad Miguel Hern\u00e1ndez, Elche
Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE)
Edificio Torregait\u00e1n. Unidad de Bioimpresi\u00f3n 3D (Estancia E10P1047)<\/p>

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