CyTOF XT mass cytometer coupled to Hyperion XTi imaging system
IDiBE features a mass cytometer (CyTOF XT) which is complemented by an imaging system (Hyperion XTi) from Standard BioTools.
The CyTOF XT allows the analysis of single cells labelled with stable metal isotopes using advanced inductively coupled plasma technology. With 135 detection channels, this instrument is capable of simultaneous multiple markers, maximising the information obtained per cell.
This system can be coupled to the Hyperion XTi high-throughput imaging system, capable of analysing more than 40 protein markers simultaneously. The use of these metallic markers enables a spatial resolution of 1 µm in tissues and cell smears, resulting in high spatial and parametric definition of cells in situ.
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CyTOF XT |
Hyperion XTi |
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Mass range |
75 – 209 uma |
75 – 209 uma |
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Channels |
135 |
135 |
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Simultaneous markers |
>45 |
>40 |
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Sample format |
Pellet or suspension |
Cell monolayer 4-7 µm |
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Throughput |
500 events/second |
800 pixels/second |
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Spatial resolution |
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Preview Mode: 25 µm Tissue Mode: 5 µm Cell Mode: 1 µm |
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Instrument response |
500 Tb159 dual counts/EQTM Six Element Calibration Bead |
Preview Mode: ≥700mm2/h Tissue Mode: ≥50mm2/h Cell Mode: ≥2mm2/h |
The acquisition of this equipment has been possible thanks to the Grant EQC2021-007485-P funded by MICIU/AEI/10.13039/501100011033 and the “European Union NextGenerationEU/PRTR”.
Contact: Marta Rubio Camacho (e-mail: marta.rubioc@umh.es; phone: +34 965 22 26 88)
